Abstract
BackgroundThe expression of SIN3A is closely correlated with electroacupuncture (EA) treatment efficacy of scopolamine-induced amnesia (SIA), but its underlying mechanisms remain to be further explored.MethodsQuantitative real-time PCR was performed to analyze the expression of candidate microRNAs (miRNAs) and SIN3A mRNA in a rat model of SIA. Western blot was carried out to evaluate the differential expression of SIN3A proteins under different circumstances. Luciferase assay was used to explore the inhibitory role of certain miRNAs in SIN3A expression. A novel object recognition (NOR) test was performed to assess the memory function of SIA rats undergoing EA treatment. Immunohistochemistry was carried out to evaluate the expression of SIN3A in the hippocampus of SIA rats.ResultsRno-miR-183-5p, rno-miR-34c-3p and rno-miR-210-3p were significantly up-regulated in SIA rats treated with EA. In addition, rno-miR-183-5p and rno-miR-210-3p exerted an inhibitory effect on SIN3A expression. EA treatment of SIA rats effectively restored the dysregulated expression of rno-miR-183-5p, rno-miR-210-3p and SIN3A. EA treatment also promoted the inhibited expression of neuronal IEGs including Arc, Egr1, Homer1 and Narp in the hippocampus of SIA rats. Accordingly, the NOR test also confirmed the effect of EA treatment on the improvement of memory in SIA rats.ConclusionIn summary, the findings of this study demonstrated that scopolamine-induced amnesia was associated with downregulated expression of miR-210/miR-183 and upregulated expression of SIN3A. Furthermore, treatment with EA alleviated scopolamine-induced amnesia in rats and was associated with upregulated expression of miR-210/miR-183 and downregulated expression of SIN3A.
Highlights
Scopolamine is a strong amnestic compound with the ability to block the activity of muscarinic acetylcholine receptors to impair the memory and learning functionsYe et al Mol Med (2020) 26:107 generalization of odor representations in the rat olfactory cortex (Wilson 2001).Electroacupuncture (EA) was initially used in European countries such as Italy and France in the nineteenth century (Xiang et al 2017)
Up‐regulation of rno‐miR‐183‐5p, rno‐miR‐34c‐3p and rno‐miR‐210‐3p in rats treated with EA The expression of a group of miRNA candidates was Immunohistochemistry assay Samples of collected rat hippocampus tissues were sliced into 4 μm sections, fixed using PBS containing 4% paraformaldehyde, and quenched with 3% H2O2 to remove the activity of endogenous peroxidase
After washing and further incubation with biotinlabeled secondary antibodies (1:1000 dilution, Abcam, Cambridge, MA), the slides were incubated with horseradish peroxidase (HRP)-tagged avidin/streptavidin and counter-stained with 4′,6-diamidino-2-phenylindole (DAPI) before the positive expression of SIN3A was analyzed underneath a microscope
Summary
Electroacupuncture (EA) was initially used in European countries such as Italy and France in the nineteenth century (Xiang et al 2017). At this moment, EA became a popular type of traditional Chinese medicine in the treatment of a wide range of medical conditions (Wang and Wu 2017). The electro stimulations of anatomical positions generated by EA can affect various physiological processes, such as angiogenesis, tissue regeneration and neuron activation, which may be promoted by the increased levels of expression of some genes in the nervous system induced by EA (Pomeranz 1996; Melzack and Wall 1965). The expression of SIN3A is closely correlated with electroacupuncture (EA) treatment efficacy of scopolamine-induced amnesia (SIA), but its underlying mechanisms remain to be further explored
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