Electrical stimulation rescues dopaminergic degeneration in dorsal raphe nucleus and enhanced hippocampal neurogenesis of vulnerable depressive rats

Abstract

Event Abstract Back to Event Electrical stimulation rescues dopaminergic degeneration in dorsal raphe nucleus and enhanced hippocampal neurogenesis of vulnerable depressive rats Sharafuddin Khairuddin1*, Wei Ling Lim2, William Wai Lun Fung1, Yasin Temel3 and Lee Wei Lim1 1 The University of Hong Kong, School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, Hong Kong (SAR) 2 Sunway University, School of Biological Sciences, Malaysia 3 Maastricht University, Departments of Neuroscience and Neurosurgery, Netherlands Electrical stimulation is a potential treatment for patients with depression. In this study, we investigate the effects of high-frequency stimulation (HFS) on various depressive-like behaviours using the stress resilience and vulnerable rat depression models. Our results demonstrated that animals with 3 weeks chronic unpredictable stress (CUS) induced exhibition of reduced sucrose consumption in 51% of animals, thus separating the resilience and vulnerable group of CUS-induced model. CUS vulnerable sham animals demonstrated anxiety-like behaviour, decreased motivation and increased immobility compared to that of the resilience group, implicating high susceptibility of vulnerable individuals to the CUS procedure. Interestingly, vmPFC HFS significantly reduced anxiety response, increased hedonia and motivation levels for food intake in the vulnerable group compared to the resilience group. HFS in ventromedial prefrontal cortex (vmPFC) and lateral habenula also showed reduced behavioural despair in both CUS vulnerable and resilience groups. In histochemistry, our results demonstrate that vmPFC HFS rescued the stress-induced dopamine neuron degeneration in the dorsal raphe nucleus, as well as increased hippocampal neurogenesis in stress vulnerable animals. In conclusion, these results suggest that vmPFC HFS effectively restores depressive-like behaviours by mechanisms of dorsal raphe dopaminergic neurons restoration and enhanced hippocampal neuroplasticity in the vulnerable CUS-induced model. Keywords: Dopamine, Hippocampus, Neurogenesis, dorsal raphe nucleus, in vivo Conference: 14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016. Presentation Type: O01: Postgraduate Travel Awardees Oral Session 1 Topic: 14th Meeting of the Asian-Pacific Society for Neurochemistry Citation: Khairuddin S, Lim W, Fung W, Temel Y and Lim L (2016). Electrical stimulation rescues dopaminergic degeneration in dorsal raphe nucleus and enhanced hippocampal neurogenesis of vulnerable depressive rats. Conference Abstract: 14th Meeting of the Asian-Pacific Society for Neurochemistry. doi: 10.3389/conf.fncel.2016.36.00078 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Aug 2016; Published Online: 11 Aug 2016. * Correspondence: Dr. Sharafuddin Khairuddin, The University of Hong Kong, School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, Hong Kong, Hong Kong, Hong Kong (SAR), sharafkdin@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Sharafuddin Khairuddin Wei Ling Lim William Wai Lun Fung Yasin Temel Lee Wei Lim Google Sharafuddin Khairuddin Wei Ling Lim William Wai Lun Fung Yasin Temel Lee Wei Lim Google Scholar Sharafuddin Khairuddin Wei Ling Lim William Wai Lun Fung Yasin Temel Lee Wei Lim PubMed Sharafuddin Khairuddin Wei Ling Lim William Wai Lun Fung Yasin Temel Lee Wei Lim Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.