Electric Cell-substrate Impedance Sensing in Biocompatibility Research.

Andrzej Kociubiński

doi:10.2478/joeb-2021-0019

Abstract

In this paper, the possibility of using cell culture impedance measurements to assess the biocompatibility of a material in contact with cells was analyzed. For this purpose, the Electric Cell-substrate Impedance Sensing (ECIS) method and a commercial measuring device were used. The test substrates with thin-film electrodes made of various metals were prepared using the magnetron sputtering method. The choice of metals was dictated by their varying degrees of biocompatibility. Cultures of mouse fibroblasts were cultured on the prepared substrates. The experiment showed that the complete cycle of culture from attachment and reproduction to apoptosis occurred. The results obtained indicate that it is possible to use the ECIS method to study the influence of metal on cell culture activity.

Highlights

Lab-on-a-chip systems are devices designed to miniaturize analytical or bioanalytical techniques
In this paper, the possibility of using cell culture impedance measurements to assess the biocompatibility of a material in contact with cells was analyzed
Cell culture was expected to develop properly on the produced matrices and the obtained measurement results would allow the analysis of cell activity

Summary

Introduction

Lab-on-a-chip systems are devices designed to miniaturize analytical or bioanalytical techniques. They are usually based on sensors and actuators produced in microelectronics and MEMS technologies. In biomedical and biological applications, some parts of the device must be in contact with a living system without producing an adverse effect. In the case of contact with metallic materials, noble metals, gold or platinum are usually used which provides the devices a high degree of biocompatibility. In many cases it is not necessary to use materials with high biocompatibility because the measurement time is so short that it does not affect the measurement results and at the same time does not pose a risk of adverse reactions. The possibility of using specific materials requires testing their biocompatibility, i.e. their effect on living organisms [1,2,3]

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Results

Conclusion