Elastase, Hyaluronidase and Tyrosinase inhibitor activities antiaging of Curcuma mangga Val. extract and its fractions

Abstract

Curcuma mangga Val. has bioactive compounds such as curcumin. Curcumin is a natural antioxidant and known as antiaging agent. Curcumin can stimulate the synthesis of collagen type I, inhibit melanogenesis and help to maintain correct skin hydration. This show C.mangga Val. has potential as antioxidant and antiaging agents. This research aims to find out the antiaging activities of C. mangga ethanolic extracts (CMEE) and its fractions. In this study, the antiaging activity was performed by measuring inhibition of elastase, hyaluronidase, and tyrosinase inhibitory activity assay. Ascorbic acid using as a control. In elastase inhibitory activity, ascorbic acid has the highest activity (15.53 µg/mL) but ethyl acetate fraction of C. Mangga (EACM) also has high activity (26.34 µg/mL) compared to other fractions and CMEE. In hyaluronidase inhibitory activity, ascorbic acid has the highest activity (27.67 µg/mL) but CMEE also has high activity (80.04 µg/mL) compared to other fractions, while in tyrosinase inhibitory activity EACM has the highest value (40.34 µg/mL) compared to ascorbic acid (65.99 µg/mL). In summary, C. mangga extracts have anti-hyaluronidase, while EACM also has good antiaging properties through anti-elastase and antityrosinase activity.