Collagenase, Elastase and Hyaluronidase Inhibitory Activities of Some Polyphenols Isolated from Ononidis Radix

Abstract

The genus Ononis L. which belongs to Fabaceae family, is represented by more than 75 species in Europe as well as Atlantic Islands, West Asia and North Africa. The genus is especially distributed closed to the coasts of Mediterranean Sea (Al-Khalil, 1995; Reyes-Betancort & Scholz, 2008). Ononis spinosa L. is a well known species especially in the central region of Turkey and the roots of this species is used as folk medicine against skin diseases due to its wound healing activity (Altanlar et al., 2006; Sever Yilmaz et al., 2006). In this study, in vitro collagenase, elastase and hyaluronidase inhibitory activities of five polyphenolic compounds, namely trifolirhizin, ononin, medicarpin-3-O-glucoside, onogenin-7-O-glucoside and sativanone-7-O-glucoside, were evaluated. The compounds were isolated from the ethylacetate fraction of the roots of O. spinosa and the structure of the bioactive compounds were established by MS, 1H- and 13C-NMR as well as 2D-NMR (COSY, TOCSY, HSQC, HMBC) techniques. According to the results; among the tested compounds, ononin and sativanone-7-O-glucoside were found to possess hyaluronidase and elastase inhibitory activity. At the dose of 100 μg/ml, ononin showed %31.66 hyaluronidase inhibitory activity and %41.75 elastase inhibitory activity; as the results were found to be %45.58 and %46.88 sativanone-7-O-glucoside, respectively.