Abstract
Aim: The present study aimed to identify the changes on the bladder mucosa in rats after intravesical infusion of Super-oxidized solution (SOS) (Mycrodacyn60® Mexico).
 Design: Eighteen Wistar Albino rats weighing 250-300 g were randomly categorized into three groups. Rats in Group 1 were infused with a single dose of 1.5 ml of 0.9% saline solution (SS) whereas those in group 2 were administered a single dose of 1.5 ml pH neutral SOS. Control rats in Group 3 did not undergo any procedure.
 Results: The absence of bacteria was verified before and after the administration of the solution, performing urine cultures and amplifying the 16S rRNA fragment by PCR and histopathological examinations for each bladder. The absence of bacterial DNA in the uroepithelium was demonstrated, histopathological examination revealed, that irrigation with SOS, in histological observation, no structural alterations were identified in the urothelial mucosa.
 Limitations: It was not possible to analyze the effect of SOS absorption indamage to the uroepithelium secondary to traumatic infection or injury. The effect of irrigation at different times and volumes with SOS was also not analyzed.
 Originality: It is known that SOS is useful as disinfectant in skin and serosa bacterial infections, lacking toxicity and it is effective against Escherichia coli. The use of superoxide solutions in cavities such as the bladder, has not been investigated yet.
 Conclusion: The pH-neutral SOS infused transurethral in the bladder of rats has no adverse effects on the uroepithelium, which was supported by histopathological evidence.
Highlights
The super-oxidized solution (SOS) is a neutral pH solution that has a powerful disinfectant activity,(1) antiseptic effect of SOS has been reported in patients with second and third degree burns, cleaning of superficially infected wounds.[2,3].Superoxide solutions (SOS) are electrochemically processed solutions, which are prepared using sterile water and sodium chloride (NaCl)
The pH-neutral SOS infused transurethral in the bladder of rats has no adverse effects on the uroepithelium, which was supported by histopathological evidence
Including 18 female rats which met the selection criteria and in which the absence of bacteria was verified through bacteriological culture, showed that the urine samples and bladder tissue were not contaminated with bacteria
Summary
The super-oxidized solution (SOS) is a neutral pH solution that has a powerful disinfectant activity,(1) antiseptic effect of SOS has been reported in patients with second and third degree burns, cleaning of superficially infected wounds.[2,3].Superoxide solutions (SOS) are electrochemically processed solutions, which are prepared using sterile water and sodium chloride (NaCl). There are several studies that show that SOS, possess antimicrobial activity towards multidrug-resistant strains (MDR), viruses, fungi and spores as well as reports of its use in cases of perforated appendicitis with beneficial effects,(5,6) used in patients with pelviperitonitis, demonstrated that it did not cause lesions of these tissues,(5) including an experimental study in rats about tissues of the uterus and ovary without any histopathological evidence of adverse toxic reaction and structural deterioration within endometrial glands and myometrial tissue.[6]. The present study aimed to identify the changes on the bladder mucosa in rats after intravesical infusion of SOS, based on observations by Zinkevich V. et al, in 2000.(8) We propose that in 10 minutes of exposure, with SOS, it will be enough to penetrate a bacterium, interfering with its metabolic activity and causing structural damage, it will allow evaluating the effect of SOS on urothelial tissue
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