Ekstraksi Kayu Artocarpus lakoocha: Pengaruh Metode Dan Rasio Bahan Tumbuhan-Penyari Terhadap Rendemen Ekstraksi

Jirapat Theeravit,Dwi Hartanti

doi:10.30595/pharmacy.v15i1.2813

Abstract

Artocarpus lakoocha Roxb. (Ma-haad) is a medicinal plant commonly used in Thai Traditional Medicine (TTM) for a wide array of indications. In this study, we compared yield of extraction and the Thin Layer Chromatography (TLC) profile of the heartwood of A. lakoocha extracted with the different methods and different material-solvent ratios. Extractions were carried out by maceration and reflux extraction with ethanol as the solvent, in material-solvent ratio of 1:5 and 1:3. The TLC profile was obtained from the separation of the extracts with methylene chloride/methanol (85:15) on silica gel F254 plate. The result exhibited that reflux extraction produced the highest yield of extraction and the material-solvent ratio of 1:3 generated more yield than that of 1:5. Nevertheless, the profile of TLC chromatogram of those extracts were similar.

Highlights

Methods for extraction and isolation of natural products are wellestablished nowadays
The size of particle of the extracted material did not affect the efficiency of extraction, as previous reported that particle size of powder of Andrographis paniculata did not significantly affect the yield of extraction of andrographolide (Wongkittipong et al, 2004)
The higher temperature used in an extraction process generally will increase the yield of extraction, as the use of thermal energy will increase solubilization of metabolites in the plant materials and disrupt cellular structures of the plant material

Summary

Introduction

Methods for extraction and isolation of natural products are wellestablished nowadays. Both are popular and easy to perform to extract bioactive compounds from medicinal plants These traditional extraction methods have drawbacks such as the use high temperature, consumption of large amount of solvent, long extraction time, the need to evaporate of huge amount of solvent and low yield (Sahne et al, 2016). These drawbacks are applied for maceration and reflux extraction. Isolation of a peroxidase with a wound healing properties was reported previously (Shila et al, 2015) Those metabolites were considered responsible for the pharmacological activity of A. lakoocha. The yield of extraction and Thin Layer Chromatography (TLC) profile of the extracts were compared

Method Material

Results and Discussion

Method Maceration Maceration Reflux

Conclusion