Abstract
Unbalanced interaction between plant and biotic or abiotic components is one of the sources to accelerate the presence of disease. The purpose of this study was to obtain a number of bacteria from Scleroderma citrinum extraction which was describing bacteria morphology, and the observed response of the potential bacteria S. citrinum as antagonists against the disease of Fusarium sp. The results obtained 7 species bacteria from S. citrinum ectomycorrhiza which had the ability to inhibition of different Fusarium sp., diseases. In addition, the 7 types of bacteria S. citrinum had different morphology. Bacterial morphology can be described based on their shapes, edges, colors, and surfaces. The result of the antagonistic test of bacterial isolate Scl 7 was suspected and had the potential to inhibit Fusarium sp., pathogen, in which it can be seen from the highest percentage from the percentage of inhibition of 41.11%. From the study, it has been observed that no visible exploitation by bacteria S. citrinum as the antagonist against Fusarium sp. Therefore, bacteria S. citrinum was suspected to contain antibiosis and competition (nutrient competitor) characteristics.
Highlights
Unbalanced interaction between plant and biotic or abiotic components is one of sources to accelerate the presence of disease
The purpose of this study was to obtain an amount of bacteria from Scleroderma citrinum extraction which was describing bacteria morphology, and observed a response of the potential bacteria S. citrinum as antagonists against the disease of Fusarium sp
Bacterial morphology can be described based on their shapes, edges, colors, and surfaces
Summary
Media yang digunakan pada penelitian ini terdiri dari 2 media, yaitu media PDA Pemurnian Bakteri Permurnian bakteri menggunakan metode gores dimana bakteri yang telah didapatkan dari jamur S. citrinum kemudian pembuatan biakan murni dari bakteri-bakteri tersebut dilakukan secara aseptik pada laminar air flow cabinet dengan menggoreskan bakteri menggunakan jarum ose pada media NA dalam cawan petri, selanjutnya bakteri tersebut diinkubasi pada suhu ruangan 25 °C selama ± 3 hari. Rancanangan Penelitian dan Analisis Data Rancangan yang digunakan dalam uji antagonis bakteri asal jamur S. citrinum terhadap cendawan Fusarium sp. Model matematis rancangan acak lengkap (Mattjik 2002 dalam May 2011) sebagai berikut : Yij = μ + τi + εji Yij : Respon penyakit Fusarium sp terhadap perlakuan Bakteri asal Scleroderma citrinum (1, 2, 3, 4, 5, 6, dan 7).
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