Determination of anti-microbial properties of Ocimum viride concentrations on Rhizopus stolonifer infection and germination of soybean (Glycine max L. Merill)

Abstract

Investigation was conducted to determine the anti-microbial properties of different concentrations of Ocimum viride and germination of soybean. Analysis of variance recorded a highly significant difference on the percentage occurrence of different fungal diseases at 5% probability level. Ocimum viride at 0.04 ppm had the highest mycelia growth inhibition 77.0%, while 0.00 ppm (control) recorded the lowest mycelia inhibition 1.00% of Rhizopus stolonifer. Investigation revealed that spot recorded the highest percentage occurrence 50%, scab 34%, mould 43% and blight 31.0% recording the lowest. Also the microorganisms isolated from diseased soybean were Rhizopus stolonifer, Fusarium oxysporum, Aspergillus species although the inhibition was very high on Rhizopus stolonifer, followed by Aspergillus sp, then mould when Fusarium sp was low. Also, Ocimun viride extract was observed to possess a high anti-microbial properties as it inhibited the growth of all the micro-organisms identified to be associated with the soybean sees. Result showed that seeds treated with crude extract of 0.04ppm had the highest percentage germination irrespective of the days of germination, followed by 0.03 ppm, then 0.02 ppm, when 0.00 ppm had the lowest. However, 0.01 and 0.02 ppm, respectively, recorded similar percentage germination 64%; 64% at 4 days after planting. Days of planting were highly significant on the percentage germination at 5% probability level, as germination of the soybean seeds increased with days of planting and highest percentage germination of 98% was recorded on the 6th day of planting while no germination was recorded from the 1st till the 4th day of planting.