Abstract
Summary δ 13 C values of different parts of the wheat plant were found to range between -25 and -34 °against PDP standard. For oat plants the variation of δ 13 C values appeared to be smaller. Wheat when grown at higher temperatures (approx. 30 °C) or under saline conditions showed an increase up to 2 °for the isotope discrimination. The isotope discrimination also changed with growth phase: it increased up to the 4th–5th leaf, but then usually decreased in plant parts above the 5th leaf. With both wheat and maize leaves a correlation between δ 13 C values and the relative activities of ribulosebisphosphate carboxylase and phosphoenolpyruvate carboxylase was found. Among the constituents of oat plants and of oat, wheat and maize grains, organic acids and sugars had the less negative δ 13 C values while the values for proteins and lipids were more negative, probably due to an additional carbon discrimination during biosynthesis of these substances. The results indicate that variations of δ 13 C values caused by ecological conditions can be traced back to changes in carboxylase pattern and to formation of secondary metabolites.
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