Einfluß verschiedener Wuchsstoffe and Stoffwechselgifte auf wurzelregenerierendes Gewebe von Phaseolus vulgaris L.

Abstract

Hypocotyl cuttings of Phaseolus vulgaris L. were subjected to the influence of the growth substances indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA), the inhibitor of the basipetal auxin transport 2,3,5-triiodobenzoic acid (TIBA) and the inhibitors of the oxidative phosphorylation 2,4-dinitrophenol (DNP) and sodium azide (NaN 3 ) during the phase of root regeneration. In the root regenerating tissue of the cuttings, changes in the contents of IAA and IBA and in the peroxidase and IAA oxidase activities after 1, 2, 4 and 6 days of regeneration were investigated. In all investigated tissues — including the untreated initial stage — IBA could be detected in addition to IAA. In the control cuttings and in all the treated cuttings a distinct increase in the contents of IAA and IBA became evident in the regenerating tissue after 24 hours of regeneration. This increase as due to the influence of IAA and IBA was somewhat higher than that detected in the control cuttings; TIBA effected only a slight increase. Treatment with DNP and NaN3 had a slight influence on the increase. With progressing regeneration, the contents of the growth substances decreased in all cases. With progressing regeneration, an increase in the activity of peroxidase and of IAA oxidase resulted from all treatments with the exception of that with NaN 3 .