Abstract
Since recently, immunoblots for detection and differentiation of antibodies against nuclear antigens (ENA), other nuclear antigens and cytoplasmic antigens, blotted on nitrocellulose strips (Western blot technique) became commercially available. The aim of our analysis was to compare the specifity and sensitivity of four different immunblot systems (blots A, B, L, V). We examined 20 sera of patients with various clinically confirmed connective tissue diseases. The sera contained a total of 32 different antigens. The antibodies in the sera were defined by double immunodiffusion (Ouchterlony) test, immunofluorescence (HEp2 cells) and by comparison with WHO reference sera.
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