Abstract
A new immunodiffusion (ID) test for antibodies to bovine leukemia virus (BLV) antigens was established. This test, refered to as the tannic acid enhanced, indirect, double immunodiffusion (IID-T) test, includes the following steps: (a) double micro-immunodiffusion using diluted references reagents, (b) treatment of the gel plate with antiglobulin serum, (c) treatment of the gel plate with 1% tannic acid. The IID-T test has proved to be eight times more sensitive than the double immunodiffusion test (ID) commonly used for anti-BLV. Diagnostic efficiency at individual levels of the IID-T test for anti-gpBLV is higher than this parameter of the ID test for anti-gpBLV and radioimmunoassay (RIA) for anti-p24BLV. The IID-T test is simple, reproducible, and more economic than the ID test in the amount of the reference reagents required. The IID-T test is more reliable than the ID test especially when weak, low titer sera are tested. Thus, the IID-T test seems to be suitable for large scale serodiagnosis of BLV infection in cattle.
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