Efficient plant regeneration through somatic embryogenesis in sugarcane (Saccharum officinarum L.) cultivar CPF-237

Abstract

-1 2,4dichlorophenoxyacetic acid (2,4-D) and 2.0% sucrose. Almost seven-weeks old proliferated calluses were sub-cultured on MS 3 [MS, 0.4 mg L -1 kinetin (kin), 0.5 mg L -1 benzyleaminopurine (BAP), 0.3 g L -1 casein hydrolysate, 3% sucrose] medium for somatic embryogenesis under dark condition for fourweeks. Greenish plantlets were regenerated (8.0 plantlets per callus) on MS 5a (MS, 0.2 mg L -1 kin, 0.3 mg L -1 BAP, 3.0% glucose) medium in six-weeks under light conditions. Regenerated plantlets were not variant morphologically and rooted on MS 7 (MS, 0.1 mg L -1 indole-3-butyric acid (IBA) medium in 1½ weeks.