Efficient nitrogen removal in a modified sequencing batch biofilm reactor treating hypersaline mustard tuber wastewater: The potential multiple pathways and key microorganisms

Abstract

This study first compared the efficiencies and mechanisms of the nitrogen removal in an aerobic sequencing batch biofilm reactor (SBBR) treating mustard tuber wastewater from high salt (30 gNaCl L−1) to ultra-high salt (70 gNaCl L−1). High-efficiency maintaining of nitrification was observed. Despite of high BOD5/TN (5.5–9), distinct denitrification decline for lack of carbon in response to salt stress was observed. Considering the high concentrations of sulfate in mustard wastewater, and the existence of sulfur-reducing bacteria (SRB) and sulfur-driven denitrifiers (DNSOB) in the aerobic SBBR, sulfate reduction-sulfur autotrophic denitrification process is a feasible idea to solve this problem. By modified to intermittent aeration mode, sulfur cycle was developed in SBBR. The average removal efficiency of COD, TN reached 85.20% and 98.56%, respectively. By batch activity tests and microbial community analysis, ammonia oxidation activity by ammonia-oxidizing archaea (AOA) was observed, and high abundance of AOA (Arch-amoA/AOB amoA: 2.38 × 102) together with ammonia-oxidizing bacteria (AOB) of Nitrosomonas_halophila (1.23%) ensured the high efficient nitrification. After running mode change, specific sulfur-driven NO3−-N reduction rate increased and the abundance of dominant SRB and DNSOB rose from 3.95% to 10.79% and 2.22% to 9.95%, respectively. The sulfate-reducing process during anaerobic phase provided electron donors for subsequent autotrophic denitrification, making outlet NO3−-N concentrations reduced from 18.26 mg L−1 to 1.93 mg L−1. The sulfur activity batch test showed that 73.80%∼80.92% of sulfate were circulation utilized, and rest of them conversed to the gaseous H2S and S0. In addition to DNSOB, anoxic denitrifier of Halomonas (22.91%), aerobic denitrifier of Phaeodactylibacter (2.75%) and endogenous denitrifier of Defluviicoccus (3.18%) were also dominant heterotrophic bacteria (all halophilic or halotolerant) in the intermittent aeration SBBR. Batch activity tests and periodic laws have also verified the existence of corresponding denitrification pathways. This study shows that the enrichment of special halophilic functional bacteria with multiple nitrogen removal pathways is a good idea for the efficient treatment of high-concentrated hypersaline industrial wastewater.