Abstract
A simple and efficient method of purification of progesterone from plasma extracts on Sephadex'LH-20 column is described. The Chromatographic system can be applied to any ligand assay for the determination of progesterone. Progesterone emerges as a compact peak well separated from related compounds. A visual dye which has the same mobility as progesterone was used to monitor the steroid fraction. This dye does not interfere with the binding assay. If redistillated solvents are used in all steps, the nonspecific blank can be eliminated. The complete absence of the procedural blank is a prerequisite for accurate determinations of plasma progesterone in man, postmenopausal woman or the follicular phase of the normal menstrual cycle.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call