Efficient hydrogen production in single-chamber microbial electrolysis cell with a fermentable substrate under hyperalkaline conditions

Abstract

Hydrogen production from food waste is of great significance for energy conversion and pollution control. The aim of this study was to investigate the glucose fermentation from food waste and hydrogen (H2) production in the single-chamber microbial electrolysis cell (MEC) under hyperalkaline conditions. Single-chamber MECs were tested with 1 g/L glucose as substrate under different pH values (i.e., 7.0, 9.5, and 11.2) and applied voltages (i.e., 0.8, 1.2, and 1.6 V). With pH increase from 7.0 to 11.2, H2 production with methanogenesis inhibition was significantly improved in the MEC. At pH of 11.2, the maximum current density reached 180 ± 9 A/m3 with the H2 purity of 93.3 ± 1.2% and average H2 yield of 7.72 ± 0.23 mol H2/ mol glucose under 1.6 V. Acetate from glucose fermentation was the largest electron sink within 12 h. Methanobacterium alcaliphilum dominated the archaeal communities with the relative abundance of > 99.0% in the cathodic biofilms. The microbial communities and mcr A gene copy numbers analyses showed that high pH enhanced the acetate production from glucose fermentation, inhibited syntrophic acetate-oxidizing with hydrogenotrophic methanogenesis in the anodic biofilms, and inhibited hydrogenotrophic methanogenesis in the cathodic biofilms. Our results of hyperalkaline conditions provide a feasible way to harvest H2 efficiently from fermentable substrates in the single-chamber MEC.