Breaking the loop: Tackling homoacetogenesis by chloroform to halt hydrogen production-consumption loop in single chamber microbial electrolysis cells

Abstract

The presence of homoacetogenesis and hydrogen production-consumption loop significantly hinders the efficient hydrogen recovery in single chamber microbial electrolysis cells (MECs). However, no effective inhibition method has been proposed against homoacetogens hitherto. In this study, we demonstrated the effectiveness of chloroform as a homoacetogen inhibitor in MECs. In small MECs (320 mL) operated in batch mode, chloroform with concentrations of 0.02% (v/v) and 0.03% (v/v) ceased homoacetogenesis with non-fermentable substrate (acetate), enhancing hydrogen yield and cathodic hydrogen recovery from 21% and 14% to 94% and 90%, respectively. Electrochemical performance was not significantly affected as current density (based on anode surface area) only decreased from 18 A/m2 to 13 A/m2 with chloroform concentration increased from 0% (v/v) to 0.03% (v/v). However, when using a fermentable substrate (glucose), the inhibition against homoacetogens by 0.01% (v/v) chloroform led to a significant decrease in current density possibly due to the decreased production of acetate, which reduced the substrate concentration for exoelectrogenesis. Nevertheless, in a larger MEC (10 L), using an immobilized sludge culture and higher glucose concentration (56 mM), 0.02% (v/v) chloroform enhanced hydrogen production rate from 0 L/L/D to 4.9 L/L/D and current density from 12-16 A/m2 to 18–21 A/m2. Microbial community analysis revealed that the homoacetogenic Acetobacterium spp. was eliminated in the cathodic biofilms and planktonic cells by chloroform. Future improvement of this method, through reducing the residual chloroform concentration and developing more environment-friendly inhibitor using similar mechanisms, may lead to the practical application in MECs.