Efficient Detection of α-, β-, and γ-Hexabromocyclododecane Isomers and Their Hydroxylated Metabolites in Poultry Tissues Based on Dispersive Solid Phase Extraction Using an Enhanced Lipid-Removing Material Combined with UPLC-MS/MS

Abstract

A simple, quick, and efficient method based on dispersive solid phase extraction combined with ultra-high-performance liquid chromatography tandem mass spectrometry has been constructed for the detection of α-, β-, and γ- hexabromocyclododecane isomer and their hydroxylated metabolites in poultry tissues. Four different samples including poultry muscle, heart, fat, and liver were extracted with acetonitrile and cleaned up through dispersive solid phased extraction using enhanced lipid-removing material or the combination of primary secondary amine +C18+Z-sep. By analysis with UPLC mass spectrometry in full scan mode, it was shown that more co-eluting interference was removed by the former material than by the latter in four matrices. For α-, β-, and γ-hexabromocyclododecane isomer in poultry tissues analyzed by constructed method, they were quantitated using internal standards and validated at the fortification levels of 1, 10, and 50 μg kg−1. Satisfied recoveries ranging in 80.2–99.6% were obtained for three isomers with relative standard deviation ranging in 3.1–13.7%. Good recoveries were also obtained for monohydroxyl-hexabromocyclododecane, dihydroxyl-hexabromocyclododecane, monohydroxyl-pentabromocyclododecene, and monohydroxyl-tetrabromocyclododecadiene in spiked poultry liver samples.