Efficient co-production of xylo-oligosaccharides and fermentable sugars from sugarcane bagasse by glutamic acid pretreatment

Abstract

In the production of xylo-oligosaccharides (XOS) by organic acid pretreatment, it is often difficult to isolate organic acids from XOS. Here, an acidic amino acid, glutamic acid (GA), was used to pretreat sugarcane bagasse (SCB) to prepare XOS and fermentable sugars. The effects of GA concentration, hydrolysis temperature, and pretreatment time on the yield and polymerization distribution of XOS were investigated. After hydrolysis by 0.2 M GA at 140 °C for 30 min, the maximum yield of X2-5 was 53.3%, and the concentrations of xylose and furfural were 1.8 g/L and 0.1 g/L, respectively. Meanwhile, GA increased the pore size and porosity of SCB as well as the number of functional groups of amino acid residues, which improved the enzymatic efficiency and the maximum yield of glucose was 95.3%. Thus, GA pretreatment provides a more economical, environmentally friendly and sustainable method for the co-production of XOS and glucose from SCB.