Efficiency of Phenazine Compound Produced by Rhizospheric Pseudomonas fluorescens against Few Pathogenic Bacteria Isolated from Ear Infections

Abstract

The most typical bacterial infection in children of pediatric age is acute otitis media. Because resistance to these microorganisms grows, management expenses for each pathogen must be specifically adapted to that pathogen. Pseudomonas fluorescens isolated from the rhizosphere rice soil revealed a higher level of antibacterial activity against ear infection pathogenic bacteria than other species of Pseudomonas. Phenazine was extracted P. fluorescens by using organic solvents such as benzene with 3.76 μg/ml then characterized with thin layer chromatography and gave a band with a retardation factor (RF) of 0.64 based on the compound’s mobility on the silica plate. In comparison to the antibacterial activity of crude phenazine, the purified phenazine revealed the highest activity against Staphylococcus aureus at 32 mm, and the lowest activity was recorded on bacteria P. aeruginosa with an 18 mm diameter of inhibition. This leads to the encouragement of the use of phenazine compound for the treatment of the increased ear infections due to multi-resistance by causing pathogens.