Efficiency of Dimethyl Sulphoxide and Ethylene Glycol on Subsequent Development of Vitrified Awassi Sheep Embryos

Omar R Mardenli,Ms Mohammad,Ha Hassooni

doi:10.14334/jitv.v25i2.2459

Omar R Mardenli, Ms Mohammad + Show 1 more

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https://doi.org/10.14334/jitv.v25i2.2459

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Abstract

The use of cryoprotectants in vitrification would reduce the critical damages to the embryos, thus increase the survival rates. This research was conducted in the laboratory of reproductive biotechnology at the faculty of Agriculture of Aleppo University. The study aimed to evaluate the viability and survivability of early Syrian Awassi embryos under the influence of dimethyl sulphoxide (DMSO) and ethylene glycol (EG) following vitrification. Embryos were vitrified in three solutions of cryoprotectants (A: DMSO (3 ml), B: EG (3 ml), and C which was composed of a combination of DMSO (1.5 ml) and EG (1.5 ml)). After thawing, embryos that had been vitrified in C solution achieved the highest rates of cleavage (P&lt; 0.01) comparing with A and B solutions for 2-16 cell stage (50.00% Vs 30.77% and 36.36%), morula (9.00% Vs 44.44% and 40.00%) and blastocyst stage embryos (92.86% Vs 58.33% and 50.00%) respectively. Down to the hatching blastocyst stage, 2-16 cell stage vitrified embryos in C solution achieved an encouraging rate comparing with A and B solutions (39.20% Vs23.08% and 22.73% respectively). The rates of arrested embryos decreased significantly (P&lt; 0.05) after thawing across the three solutions especially the morula and blastocyst stage (0.00 and 3.70% respectively) (C solution). No significant differences were observed in the three types of embryos across all stages and solutions despite the large range among these rates. Given the apparent benefit of the participatory effect of cytoprotectants, it is advised to use a mixture of DMSO and EG (1:1) in vitrification of ovine embryos.

Highlights

Cryobiology is considered the most important science in embryo technology, especially the In Vitro Embryo Production applications (IVEP).Despite the great progress achieved by this technology in the field of farm animal industry, there are still some outstanding issues that need solutions
Our results show that the total rates of survived Awassi sheep embryos in different stages of embryonic development vitrified in C solution which composed of a combination of dimethyl sulphoxide (DMSO) and ethylene glycol (EG) cryoprotectants was greater (P < 0.01) compared to those vitrified in the tow solutions A and B: 76.3 % versus 44.1 % and 42.4% respectively
The survival rate of 2-16 cell stage embryos vitrified in C solution was slightly greater compared to those vitrified in the two solutions A and B: 50% versus 30.8 % and 36.4 % respectively, but these differences were not significant (Table 2)

Summary

Introduction

Cryobiology is considered the most important science in embryo technology, especially the In Vitro Embryo Production applications (IVEP).Despite the great progress achieved by this technology in the field of farm animal industry, there are still some outstanding issues that need solutions. Cryobiology is considered the most important science in embryo technology, especially the In Vitro Embryo Production applications (IVEP). JITV Vol 25 No 2 Th. 2020:60-67 important of which is the decrease in the survivability rates of frozen embryos during the blastomere stage, the high accumulated content of lipids (especially triacylglycerides) in embryonic cells, which have harmful effects (Palasz et al 2008). Some considerations were identified in the programmed slow freezing, Thompson et al (2011) indicated that subjecting embryos to a rate of 1 °C min–1 is considered a typical cooling rate for mammalian embryos. Equipment, and multiple steps of slow freezing, it has been observed a decrease in both survival and implantation rates (Bromfield et al 2009)

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