EFFICIENCY OF COMPLEX TECHNOLOGICAL SOLUTIONS FOR GROWING FISH TO INCREASE RESISTANCE TO THE INFLUENCE OF ABIOTIC AND BIOTIC FACTORS UNDER THE INFLUENCE OF CLIMATE TRANSFORMATIONS

Abstract

The purpose. To substantiate the effectiveness of using the method of rearing fish at the early stages of ontogenesis before stocking water bodies using a scientific-experimental method. Methodology of the study is based on the functional status of the fish organism. The experimental part of the work was performed on the basis of the Department of Aquatic Bioresources and Aquaculture of the Kherson State Agrarian and Economic University (Ukraine), laboratory of the "Kherson Production and Experimental Plant for Breeding of the Ordinary Fish" State Institution and "Aquaculture Perspectives" Scientific Research Laboratory, "Scientific Research Laboratory of Physiological and Biochemical Research of S. Pentelyuk", Scientific Research Laboratory on ecological and chemical analysis and water monitoring of Public higher education institution Kherson State Agrarian and Economic University (KSAEU, Ukraine). For studies of the main parameters that were studied, standard methods were used [1–4]. The blood from heart and tail vein was obtained using Pasteur needle and heparinized syringe. For biochemical studies, apart from blood plasma, the muscle part was also collected. They were also frozen in ThermoMix and stored for further research. Using a digital camera and Micromed microscope. Muscle protein content was determined by the Lowry method. Biochemical studies of biological material were performed using ULAB 102 spectrophotometer. Catalase activity was determined by 219Chapter «Agricultural sciences»the spectrophotometric method, based on the ability of hydrogen peroxide to form a stable color with the reagent. Biochemical analysis of samples to study the level of activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total protein content, and glucose concentration was carried out in the laboratory of the Department of Aquatic Bioresources and Aquaculture of KSAEU on a Humalyzer 3000 analyzer using standardized Human GmbH kits. Against the background of the outlined parameters, observations of the ethology of hydrobionts were carried out during the day, with the recording of certain details as necessary. All manipulations of the object of scientific research were carried out in accordance with the rules of the "European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes" (Strasbourg, 1986). Results. The results of the research work on the comprehensive study of the leading parameters of the growth efficiency with the use of optimization elements are presented. Positive results were obtained on the speed of development of young carp in polyculture with carp against the background of activation of metabolic processes in the body. The implementation of technological elements for optimizing the growth of hydrobionts in the early stages of ontogenesis helps to increase the viability of young and ensures the efficiency of stocking reservoirs with such young carp. The proposed biologically active substances in the composition of the ration reasonably provide the functionality of a micro-additive against the background of elements of resource-saving technology in the scheme of a modular fish farming system. Practical implications. The method of growing carp presented in the article before stocking with fish helps to increase growth parameters, increase resistance to the influence of abiotic and biotic factors. Value/originality. The use of resource-saving elements in the technology of growing fish. The uniqueness provides innovative solutions for optimizing the technology of growing carp and silver carp.