Efficiency and potential synthesis of pyrimido [4,5-d] pyrimidine derivatives by iodine catalyst as antioxidant agent

Abstract

Pyrimido [4,5-d] pyrimidine is an uracil compound that has similarities to the structure of pteridine and purines and has significant bioactivity. The aim of this research is to synthesize pyrimido [4,5-d] pyrimidine derivatives and test their activity as antioxidant. The reaction was carried out with the support of the iodine catalyst to get an effective reaction. Experimental evidence in support of the formation products was confirmed using FTIR, UV-vis Spectrophotometer, and GC-MS. The results of the analysis produced four pyrimido [4,5-d] pyrimidine derivatives, 5-Styryl-5,8-dihydro-1H,6H-pyrimido[4,5-d]pyrimidine-2,4,7-trione (compound 1), 5-Styryl-7-thioxo-5,6,7,8-tetrahydro-1H-pyrimido[4,5-d]pyrimidine-2,4-dione (compound 2), 5-Phenyl-5,8-dihydro-1H,6H-pyrimido[4,5-d]pyrimidine-2,4,7-trione (compound 3), and 5-Phenyl-7-thioxo-5,6,7,8-tetrahydro-1H-pyrimido[4,5-d]pyrimidine-2,4-dione (compound 4). Reaction optimization was conducted by varying reaction time, reaction temperature, amount of catalyst and solvent. The optimum conditions of the reaction were obtained under conditions of 10 % mol of catalyst, temperature 70 °C, for 4 h with a water solvent. Compound 1 has a better activity than 3 other compounds with an IC50 value of 10.11 ppm.