Efficacy of the New Hemostatic Composite Based on Sodium Gel Carboxymethylcellulose and Aminocaproic Acid: Experimental Application 
 after Partial Splenectomy in Rats

Abstract

Backgraund. Spleen surgery is mainly aimed to achieve effective hemostasis. The increasing popularity of topical hemostatic drugs is limited by their common side effect associated with the development of adhesions.The aim of study was to develop a new hemostatic drug with anti-adhesion activity. To achieve this, the authors have investigated 6% sodium carboxymethylcellulose gel with the addition of 5% aminocaproic acid. Materials and methods. The efficacy of the sample was studied experimentally in laboratory animals (rats, n = 87). The time of bleeding arrest, the amount of blood loss, the severity of the adhesion process, morphological and histological changes in tissues in the area of material implantation were assessed.Results. The study results evidenced that the new hemostatic drug reduced bleeding time by 68% (222.27 sec) and blood loss by 69% (285.6 mg) (p 0.01) after partial splenectomy, and prevented the development of adhesive process compared to the results obtained in the animals of the control group. On the 3rd day of the experiment application of the sample demonstrated that adhesions were completely absent, the coefficient of the severity of the adhesive process was 0 points; on the 7th and 14th days it was minimal - 0.481 and 0.486 points, respectively (p 0.05). A morphological study showed that the use of the new composite reduced the white pulp immunological reactivity associated with postoperative inflammatory processes, which is manifested by smaller lymphoid follicles and lower CD4 + T-lymphocytes expression.Conclusions. Thus, 6% sodium carboxymethylcellulose gel with the addition of 5% aminocaproic acid is an effective combined hemostatic and anti-adhesion agent that can be effectively applied in spleen surgery.