Simulation of a Trophic Purulent Wound: an Experimental Study

Abstract

Background. Long-term non-healing wounds are one of the common complications of multiple diseases, injuries and surgical interventions. In order to optimize their treatment options, experimental simulation of the wound process are created and improved.The aim of study was to develop an experimentally simulated trophic purulent wound, and to evaluate the potential of its use to study the impact of various factors on the wound process.Materials and methods. The trophic purulent wound simulation was performed on 80 nonlinear albino rats. Experimental wounds were formed using a silicone disk, the inner edges of which were sutured to a round wound formed in the interscapular region of the animal. Next, the silicone disc was sutured to the skin along the outer edge and additional sutures were placed around the disc to enhance tissue ischemia. The fascia at the bottom of the wound was incised, the bottom of the wound was crushed with a clamp, and a bacterial culture was injected into the wound defect. The developed model was evaluated visually, using thermometry, luminescent analysis, planimetry, microbiological, cytological and morphological tests.Results. The features of the simulated wound allowed achieving the size and protection similar to experimental wounds, and forming trophic disorders in the tissues, In 48 hours, a picture of a complicated purulent process was observed in most animals. The data obtained during the analysis of the proposed experimental model confirmed its quality, simplicity and reproducibility.Conclusion. The proposed approach can be recommended to study protracted wound processes and various factors affecting them.