Abstract

Control potential of 10 Beauveria bassiana isolates, isolated from Hypera postica (Gyllenhal) (Coleoptera: Curculionidae) and Gonioctena fornicata (Brüggemann) (Coleoptera: Chrysomelidae), collected from alfalfa fields in Tokat province, Turkey, was evaluated against H. postica larvae under laboratory conditions. Concentration-response tests were carried out using the concentrations (1 × 103, 1 × 105, 1 × 107, and 1 × 109 conidia/ml) of isolates GN-23, GN-4, HP-30, and HP-6, which performed more than 95% efficacy in screening tests (1 × 107 conidia/ml) 5 days post treatments. LT50 and LT90 values at 1 × 109 conidia/ml were determined. According to the obtained results, H. postica larvae were susceptible to all the tested B. bassiana isolates.

Highlights

  • Alfalfa (Medicago sativa L.) is one of the well-known and widely used forage crops in the world

  • The objective of this study was to determine the efficacy of 10 local B. bassiana isolates, isolated from H. postica and Gonioctena fornicata, collected from alfalfa fields in Tokat province, Turkey, against larvae of the alfalfa weevil under laboratory conditions

  • The ten entomopathogenic fungi (EPF) isolates tested against H. postica at 1 × 107conidia/ml caused 100% mortality 7 days post treatment in almost all isolates (Table 2)

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Summary

Introduction

Alfalfa (Medicago sativa L.) is one of the well-known and widely used forage crops in the world. Alfalfa is attacked by a numerous number of insect species that cause considerable damage and reduce forage yield. Alfalfa weevil Hypera postica (Gyllenhal) (Coleoptera: Curculionidae) is one of the most important pests that attack this crop. Both adults and larvae feed on alfalfa foliage, but the larvae cause the majority of the damage. Injured leaves dry very quickly giving the field a grayish to whitish cast. This pest can be found in all alfalfa production areas of Turkey and worldwide (Cook et al 2004 and Atanasova 2012)

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