Efficacy of low-dose local clindamycin in different times for microbial decontamination of autogenous particulate bone graft

Hassan Mohajerani,Faramarz Masjedian,Reza Tabrizi,Fatemeh Latifi,Gholamreza Irajian

doi:10.1186/s40729-020-00263-1

Abstract

BackgroundClindamycin in low concentration (20 μg/mL) is safe for vitality and osteogenic potential of bone cells. The aim of this study was to evaluate the efficacy of local clindamycin (20 μg/mL) in two different exposure times, for microbial decontamination of particulate bone graft, collected during implant site preparation.This non-randomized parallel-group study was conducted on samples from 17 patients. The particulate bone collected during implant site preparation was divided into three portions by weight: in group S1, the particulate bone was immersed in thioglycolate broth without any antibiotic treatment; in group S2, the collected particulate bone was irrigated with 100 mL clindamycin solution (20 μg/mL); and in group S3, the collected particulate bone was soaked in one ml clindamycin solution (20 μg/mL) for 3 min. Samples in the three groups were cultured in aerobic and anaerobic media and species and CFU count of isolated bacteria were determined.ResultsAnalysis of the data demonstrated a significant difference among the three groups in the mean count of total microorganisms (P = 0.001). The difference in the mean count of anaerobic and aerobic microorganisms in the three groups was statistically significant as well (P = 0.001). Pseudomonas aeruginosa was the only microorganism that was not affected with the mentioned antibiotic.ConclusionsLocal use of low-dose clindamycin (20 μg/mL)—irrigation or 3 min immersing—is effective for the decontamination of particulate bone grafts.

Highlights

Clindamycin in low concentration (20 μg/mL) is safe for vitality and osteogenic potential of bone cells
Regardless of the time factor, all the microbial species population reduced after decontamination with clindamycin solution (20 μg/mL), except Pseudomonas aeruginosa (Table 2)
Comparison of microorganisms colony-forming units (CFU) revealed a statistically significant reduction in nine microbial species between three groups. Pairwise comparison of these nine species demonstrated that the only microorganism that has not been significantly reduced after irrigation with clindamycin solution (20 μg/mL) is Klebsiella pneumoniae, and immersion in 3 min was more effective

Summary

Introduction

Clindamycin in low concentration (20 μg/mL) is safe for vitality and osteogenic potential of bone cells. The aim of this study was to evaluate the efficacy of local clindamycin (20 μg/mL) in two different exposure times, for microbial decontamination of particulate bone graft, collected during implant site preparation. This non-randomized parallel-group study was conducted on samples from 17 patients. Bone chips, derived from drilling, can serve as a suitable source for grafting without donor site morbidity [1] They can be collected during dental implant surgery using a bone. Due to the disadvantages of local betadine and chlorhexidine on wound healing, cell vitality, and osteogenic potential, using antibiotic solutions as a decontamination agent for bone grafts is wise. Selecting the correct drug in an accurate concentration and precise contact time— harmless to the cell vitality and their osteogenic potential—is important

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