Efficacy of Compound Herbal Medicine Tong-Xie-Yao-Fang for Acute Radiation Enteritis and Its Potential Mechanisms: Evidence from Transcriptome Analysis.

Cheng Yang,Jiayue Yang,Min Xia,Yang Jiao,Jianhong Wei,Qiang Zhan,Andrei Surguchov

doi:10.1155/2020/5481653

Cheng Yang, Jiayue Yang + Show 5 more

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https://doi.org/10.1155/2020/5481653

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Abstract

Acute radiation enteritis (ARE) is a common complication with radiotherapy for pelvic and abdominal malignancy. This research is designed to investigate the efficacy of Tong-Xie-Yao-Fang (TXYF) on ARE and to explore the underlying mechanisms by microarray analysis. The ARE rat model was established by a single abdominal irradiation with a gamma-ray dose of 10 Gy. Next, the ARE rats were treated with distilled water, TXYF, and glutamine by gavage for 7 consecutive days according to the scheduled groups. For each group, the jejunal tissue was taken at 6 h after gastric lavage. The morphology of intestinal tissue was observed by hematoxylin and eosin (H&E) stain under a light microscope. The height of the villus and the thickness of the whole layer of the TXYF-treated groups were significantly ameliorative than that of the model control group. The transcriptome analysis was produced using the Agilent SurePrint G3 Rat GE V2.0 microarray. A total of 90 differentially expressed genes (DEGs), including 48 upregulated genes and 42 downregulated genes, were identified by microarray and bioinformatics analysis. Protein–protein interaction (PPI), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) were conducted to explore the possible mechanisms of DEGs taking part in the TXYF-mediated therapeutic process for ARE. In conclusion, we reveal that TXYF has a protective effect on the intestinal tissue of rats with ARE and summarize several DEGs, suggesting the possible mechanisms of TXYF-mediated efficacy for ARE.

Highlights

In recent years, the clinical consensus for radiation therapy of tumors has been reached, and treatment is standardized gradually [1, 2]
TXYF was prepared with Rhizoma Atractylodis Macrocephalae, Radix Paeoniae Alba, Pericarpium Citri Reticulatae, and Radix Saposhnikoviae, which were purchased from the Wuxi Hospital of Traditional Chinese Medicine and composed in 6 : 4 : 3 : 2 proportions
Further quantitative analysis confirmed the successful establishment of the acute radiation enteritis (ARE) rat model

Summary

Introduction

The clinical consensus for radiation therapy of tumors has been reached, and treatment is standardized gradually [1, 2]. With the growing incidence of tumors and the increasing popularity of radiotherapy, increasing numbers of patients inevitably develop acute radiation enteritis (ARE) after radiation therapy for pelvic and abdominal malignancies [3]. ARE is a common intestinal complication during and after radiotherapy for abdominal and pelvic malignancies. Western medicine treatment can achieve certain effects in clinical practice, the overall effect is still not satisfactory. As one of China’s traditional medical treasure houses, traditional Chinese medicine has unique advantages in the treatment of gastrointestinal diseases [6,7,8].

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