Abstract
Giardia duodenalis, formerly known as Giardia lamblia, is an important zoonotic protozoan parasite. It mainly infects the intestines of humans, dogs, cats and domestic animals, causing diarrhea, abdominal pain, indigestion and weight loss. At present, all the clinical drugs for the treatment of Giardia have problems such as side effects and drug resistance to varying degrees, and the development of new drugs for the treatment of Giardia is still a hot issue. There is growing interest in using probiotics as an anti-intestinal parasite strategy. The present study aimed to assess the effect of supernatants of Bifidobacterium Animalis Subsp. lactis BB-12 on giardia the growth of giardia trophozoites. In this study, the Bifidobacterium animalis subsp. lactis BB-12 were cultured in BBL liquid medium, and the effects of the supernatants on the growth and adhesion of trophozoites of Giardia were observed. The results showed that the growth of Giardia flagellate was significantly inhibited by the supernatant. The influence of the supernatant on the morphology of the trophozoites was observed by microscope, and it was found that the surface of the trophozoites was uneven, the shape was atrophied, the surface cell membrane was broken to some extent, and the contents were spilt. In summary, the results of this study suggest that the fresh-cultured supernatants of the probiotic Bifidobacterium Animalis subsp. lactis BB-12 have anti-Giardia effects.
Highlights
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Summary
$EVWUDFW Giardia duodenalis IRUPHUO\ NQRZQ DV Giardia lamblia LV DQ LPSRUWDQW ]RRQRWLF SURWR]RDQ SDUDVLWH ,W PDLQO\ LQIHFWV WKH LQWHVWLQHV RI KXPDQV GRJV FDWV DQG GRPHVWLF DQLPDOV FDXVLQJ GLDUUKHD DEGRPLQDO SDLQ LQGLJHVWLRQ DQG ZHLJKW ORVV $W SUHVHQW DOO WKH FOLQLFDO GUXJV IRU WKH WUHDWPHQW RI Giardia KDYH SUREOHPV VXFK DV VLGH HIIHFWV DQG GUXJ UHVLVWDQFH WR YDU\LQJ GHJUHHV DQG WKH GHYHORSPHQW RI QHZ GUXJV IRU WKH WUHDWPHQW RI Giardia LV VWLOO D KRW LVVXH 7KHUH LV JURZLQJ LQWHUHVW LQ XVLQJ SURELRWLFV DV DQ DQWL LQWHVWLQDO SDUDVLWH VWUDWHJ\ 7KH SUHVHQW VWXG\ DLPHG WR DVVHVV WKH HIIHFW RI VXSHUQDWDQWV RI Bifidobacterium Animalis Subsp. lactis BB-12 RQ JLDUGLD WKH JURZWK RI JLDUGLD WURSKR]RLWHV ,Q WKLV VWXG\ WKH Bifidobacterium animalis subsp. lactis BB-12 ZHUH FXOWXUHG LQ %%/ OLTXLG PHGLXP DQG WKH HIIHFWV RI WKH VXSHUQDWDQWV RQ WKH JURZWK DQG DGKHVLRQ RI WURSKR]RLWHV RI Giardia ZHUH REVHUYHG 7KH UHVXOWV VKRZHG WKDW WKH JURZWK RI Giardia IODJHOODWH ZDV VLJQLILFDQWO\ LQKLELWHG E\ WKH VXSHUQDWDQW 7KH LQIOXHQFH RI WKH VXSHUQDWDQW RQ WKH PRUSKRORJ\ RI WKH WURSKR]RLWHV ZDV REVHUYHG E\ PLFURVFRSH DQG LW ZDV IRXQG WKDW WKH VXUIDFH RI WKH WURSKR]RLWHV ZDV XQHYHQ WKH VKDSH ZDV DWURSKLHG WKH VXUIDFH FHOO PHPEUDQH ZDV EURNHQ WR VRPH H[WHQW DQG WKH FRQWHQWV ZHUH VSLOW ,Q VXPPDU\ WKH UHVXOWV RI WKLV VWXG\ VXJJHVW WKDW WKH IUHVKFXOWXUHG VXSHUQDWDQWV RI WKH SURELRWLF Bifidobacterium Animalis subsp. lactis BB-12 KDYH DQWL*LDUGLD HIIHFWV.
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