Efficacy of Aerosol Vaccination of Adjuvantd Pigeon Pox Vaccine in Pigeon

Ayatollah I Bassiouny,Heba M El Naggar,Kafafy Mh

doi:10.17582/journal.aavs/2021/9.12.2116.2123

Abstract

Efficacy of Aerosol Vaccination of Adjuvantd Pigeon Pox Vaccine in Pigeon

Highlights

Pigeon pox is a serious virus disease in pigeons especially in young pigeon as causing high percentage of mortality due to fibrino-necrotic lesion in the mucous membrane of the upper respiratory tract and the development of discrete proliferative nodular skin lesions (Hemanth et al, 2014).Pigeon pox disease is caused by pigeon pox virus (PPV) within family Poxviridae subfamily Chordopoxvirinaeand genus Avipoxvirus (Andraw, 2012)
Determintion of Ppv Vaccine Field Dose Via Aersol Spray The results of mean neutralization index (NI) and S/P ratio as well as challenge for defferent PPV vaccine field doses administrated via spray shown in Table (2) refer Protective level of antibody represented by NI and S/P ratio in all vaccinated groups while challenging the birds 3 weeks post vacciation with virulent PPV revealed that (5) birds showed post challenging pox lesions and the other 15 birds remained healthy without local or generalized post challenging lesion with protection percent of 75% in group (2) received 10 2.5TCID50/ field dose
Serum Neutralization test and indirect enzyme linked immunosorbent assay (ELISA): Serum neutralization test as well as Indirect ELISA were carried on the serum samples collected from vaccinated pigeons via spray route without and with different concentrations of adjuvanted Gel 01 ST (Gel 01) (0%, 5%, 10% and 15% respectively) PPV vaccines and feather follicle method

Summary

Introduction

Pigeon pox is a serious virus disease in pigeons especially in young pigeon as causing high percentage of mortality due to (diphtheritic form) fibrino-necrotic lesion in the mucous membrane of the upper respiratory tract and (cutaneous form) the development of discrete proliferative nodular skin lesions (Hemanth et al, 2014).Pigeon pox disease is caused by pigeon pox virus (PPV) within family Poxviridae subfamily Chordopoxvirinaeand genus Avipoxvirus (Andraw, 2012). Pigeon pox is a serious virus disease in pigeons especially in young pigeon as causing high percentage of mortality due to (diphtheritic form) fibrino-necrotic lesion in the mucous membrane of the upper respiratory tract and (cutaneous form) the development of discrete proliferative nodular skin lesions (Hemanth et al, 2014). In Egypt, first pigeon pox vaccine was prepared by adaptation of pigeon pox virus (Hungarian strain) on the chorioallantoic membrane (CAM) of 9-11 day-old embryonated chicken eggs (Helmy et al, 1967). Recently alternative PPV vaccine was prepared by using locally isolated strain attenuated, adapted and propagated on Vero cell line (Kafafy et al ., 2018)

Methods

Results

Discussion

Conclusion