Abstract
Simple SummaryHemorrhagic septicemia (HS) and mastitis are important diseases of South Asian dairy animals. Seventy S. aureus/Str. agalactiae-free lactating buffaloes (n = 45) and cows (n = 25) and fifty S. aureus/Str. agalactiae-positive lactating (early stage of lactation) buffaloes (n = 25) and cows (n = 25) were subjected to two doses of Montanide® adjuvant combined HS–mastitis vaccine with 21 days of interval. Vaccinated groups showed mean somatic cell counts and mastitis severity scores that were significantly lower (p < 0.05), whereas the milk yield was significantly higher (p < 0.05). In conclusion, this vaccine can be used as a potential preventive measure against HS and mastitis in dairy animals.Hemorrhagic septicemia (HS) and mastitis caused by Pasteurella (P.) multocida, Staphylococcus (S.) aureus and Streptococcus (Str.) agalactiae are important ailments of the dairy industry especially in South Asia. The present study evaluates the efficacy of a locally prepared hemorrhagic septicemia (HS) and mastitis combined vaccine. To this end, a total of 70 HS, S. aureus and Str. agalactiae-free lactating (early stage of lactation) buffaloes (n = 45) and cows (n = 25), and 50 lactating (early stage of lactation) cows (n = 25) and buffaloes (n = 25) positive for S. aureus/Str. agalactiae were subjected to two doses of HS–mastitis combined vaccine with an interval of 21 days. Antibody response was monitored by ELISA up to six months (180 days). Antibody titers against HS and mastitis were significantly (p ˂ 0.05) higher in vaccinated groups as compared to the non-vaccinated groups. Cumulative mean somatic cell counts and mastitis severity scores in vaccinated groups were significantly lower (p < 0.05), and milk yield was significantly higher (p < 0.05) than those in the respective non-vaccinated controls. In conclusion, Montanide®-adjuvanted HS–mastitis combined vaccine showed significant immunogenic effects in dairy cows and buffaloes. However, challenge studies remain overdue.
Highlights
Hemorrhagic septicemia (HS) and bacterial mastitis are important ailments in South Asian dairy animals [1–4]
Hemorrhagic septicemia is an infectious, fatal bacterial disease caused by P. multocida type B:2 [5] which accounts for annual losses of USD 12.4 million in the province of Punjab only
Peripheral blood and bone marrow samples from clinical cases of HS were cultured on blood, casein-sucrose-yeast (CSY) and MacConkey’s agar for isolation of P. multocida; quarter foremilk samples from clinically affected cows and buffaloes were used for the isolation of S. aureus and Str. agalactiae as per the National Mastitis Council (NMC), New Prague, Minnesota, USA guidelines [12]
Summary
Hemorrhagic septicemia (HS) and bacterial mastitis are important ailments in South Asian dairy animals [1–4]. Both diseases lead to serious economic losses in terms of mortality, production loss and treatment costs directly on farmers, whereas surveillance programs cost additional burden on the national economy [1]. Mastitis (inflammation or swelling of milk-producing organ) is another common dairy animal disease which, not fatal, causes colossal economic losses to our resource-poor dairy farmers and milk-processing industry [7]. Mastitis-affected populations of buffaloes and cattle sustain ~25% reduction in their milk yield and render the milk unwholesome for human consumption as it may contain pathogenic bacteria, toxins and other harmful substances which may not be neutralized by ultra-high temperature (UHT) treatment [7]
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