Thyroxine, Triiodothyronine and Reverse-Triiodothyronine Concentrations in Blood Plasma in Relation to Lactational Stage, Milk Yield, Energy and Dietary Protein Intake in Estonian Dairy Cows

Abstract

Average levels of thyroxine (T4), triiodothyronine (T3) and reverse-triiodothyronine (rT3) in blood plasma of 159 Estonian Red and Estonian Black and White cows were 55.2 nmol/L, 1.78 nmol/L and 0.25 nmol/L respectively. Animals were grouped according to stage of lactation. The T4 level was significantly lower during the early stage of lactation (45.1 nmol/L), compared with later stages, but increased as the stage of lactation progressed (late stage of lactation - 56.7, dry cows 64.3 nmol/L). The T3 level was significantly higher at the late stage of lactation (1.93 nmol/L) compared with the early stage of lactation (1.71 nmol/L) and level in dry cows (1.71 nmol/L). rT3 showed a trend similar to that found for T4 (lowest plasma concentration in early lactation, 0.19; highest in the dry period, 0.33; late lactation, 0.24 nmol/L). Levels of all thyroid hormones were negatively related to the daily milk yield (T4-r = -.51, rT3-r = -.47, calculated thyroid index as rT3 x T3 x T4-r = -.52, for all p < .0001; T3-r = -.32, p < .01). Plasma thyroid hormone concentrations were affected by energy and dietary protein intake. Differences were found in thyroid hormone levels between the 2 breeds and between summer and winter holding periods for Estonian Red cows, which could all be explained by differences in the feeding level and daily milk yield. It is suggested that maintaining low levels of thyroid hormones in early lactation may be one of the dairy cow's mechanisms of reducing metabolic demand.