Effects on oncostatin M in hyperglycemia–induced aortic smooth muscle cells proliferation in human

Abstract

Objective To study the effects of high blood glucose on expression of oncostatin M (OSM) in macrophages and the association of OSM with proliferation of human aortic smooth muscle cells. Methods Monocytic cell line THP–1 was incubated with PMA to induce macrophages. The human macrophage cell line–derived cytokines influenced by hyperglycemia (15 mmol/L) were screened by human cytokine antibody array, which was further confirmed by Western blot and ELISA. RT–PCR was used to determine the expression of OSM receptors GP130–OSMRβ and GP130–LIFR in human aortic endothelial cells, smooth muscle cells and macrophages. The effects of OSM on proliferation of human aortic smooth muscle cell was assessed using the XTT assay. One–way analysis of variances was used for data analysis. Results Incubation with PMA induced mature macrophages. Human cytokine antibody array showed that high blood glucose up–regulated the expression of OSM in macrophages, which was further confirmed by Western blot and ELISA. The expression of GP130–OSMRβ and GP130–LIFR was found in human aortic endothelial cells, smooth muscle cells and macrophages. XTT assay showed that OSM promoted proliferation of human aortic smooth muscle cells. Compared with 0 μg/L OSM (OD=0.468±0.032), the proliferation of human aortic smooth muscle cells was significantly increased in 5, 10, 20 μg/L OSM (OD=0.503±0.026, 0.520±0.027, 0.513±0.026, respectively) (F=10.40, P<0.05) Conclusions High blood glucose could increase the expression and secretion of OSM in macrophages. OSM may promote the proliferation of human aortic smooth muscle cells by binding with its receptors, which partly involves in the development of diabetic macroangiopathy. Key words: Diabetes mellitus; Macrophages; Muscle, smooth, vascular; Oncostatin M