Abstract
The effects of x irradiation (300, 500, and 1000 r) on mitosis and DNA synthesis in Ehrlich ascites tumor cells were studied. Dose-effect relations and time sequence of effects on the two processes were compared. The technique of H/ sup 3/-thymidine labeling of DNA with autoradiography was utilized, in conjunction with the mitotic index, to study alterations in the mitotic cycle. It was concluded that x irradiation affected cells in the G/sub 2/ and S periods so that the rate of exit from G/sub 2/ was reduced. Evidence, based on mean grain count per labeled cell and percentage of cells labeled, was found for a slight reduction in rate of DNA synthesis after irradiation. The delay in rate exit from G/sub 2/, however, was apparently a block at the end of the period. (auth)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.