Abstract
Summary Alpha-amylase activity in detached cotyledons of Pisum sativum increased gradually during incubation with distilled water, in contrast to the absence of any increase in α-amylase activity in embryo-free halfgrains of barley during incubation. We examined whether the wounding caused by cutting away the embryonic axis influenced the development of α-amylase activity in the wounded cotyledonary tissue. The larger the amount of detached cotyledons that was removed with the axis, the greater was the increase in enzymatic activity during incubation with distilled water. The development of α-amylase activity was accelerated dramatically during incubation with auxins (Hirasawa, 1989). The development of activitiy in detached cotyledons of Pisum sativum during incubation with distilled water or with 2,4-dichlorophenoxyacetic acid was inhibited in the presence of ethylene or abscisic acid. Alpha-amylase activity in detached cotyledons of Phaseolus vulgaris and Azuki angularis increased during incubation with distilled water, but 2,4-dichlorophenoxyacetic acid, abscisic acid, and ethylene had no effect on the increase in the cotyledons of these Leguminosae. These results suggest that α-amylase activity in detached cotyledons during incubation with distilled water is induced by wounding caused by removal of the embryonic axis and/or by an endogenous auxin in the cotyledons.
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