EFFECTS OF WALNUT LEAVES EXTRACT ON EFFICACY OF DAPAGLIFLOZIN IN TYPE II DIABETIC MICE

Abstract

Background: With a rising prevalence of type 2 diabetes mellitus its becoming more important than ever to find new strategies to prevent and cure the disease. Objective of this study was to assess the effects of walnut leaves extract on the efficacy of dapagliflozin in type 2 diabetic mice. Methods: It was experimental, randomized control study. A total of 50 male Balb/c mice were included in this study and randomly divided into two groups: Group 1 (Normal Control) consisting of 10 mice and Group 2 (experimental group) of 40 mice. The experimental group was further divided into 4 groups of 10 mice each following induction of type 2 diabetes: Group 2 (Disease Control), Group 3 (Ethanolic walnut leaves extract treated), Group 4 (Dapagliflozin treated) and Group 5 (Combination of dapagliflozin and ethanolic walnut leaves extract treated) for a duration of 45 days. Statistical analysis was done using SPSS-27. One-way ANOVA (post hoc Tukey test) was used to compare the means of HbA1c among groups, and p<0.05 was considered significant. Results: Mice treated with walnut leaves extract (Group 3), dapagliflozin (Group 4), and the combination of walnut leaves extract and dapagliflozin (Group 5) exhibited significantly reduced serum HbA1c levels compared to those observed in the diabetic control group (p<0.05). Conclusions: The combined administration of walnut leaves extract and dapagliflozin leads to a significant decrease in serum HbA1c levels in diabetic mice, as compared to mice treated with walnut leaves extract or dapagliflozin alone. Pak J Physiol 2013;19(4):26?8