Effects of Vitamin D and Simvastatin on Inflammatory and Oxidative Stress Markers of High-Fat Diet-Induced Obese Rats

Abdel-Moniem A Makhlouf,Rania G Ibrahim,Atef M Mahmoud,Yasmeen S Abdel Aziz

doi:10.47631/jsrmbs.v2i3.297

Abstract

Purpose: This study was aimed to evaluate the antioxidant and anti-inflammatory effects of vitamin D and Simvastatin (SIM) on a high-fat diet (HFD) induced-obese rats. Methods: 40 adult male rats were divided into four groups: control group, HFD, HFD + vitamin D, and HFD + SIM for 14 weeks. Vitamin D or SIM supplementation was done for the last 6 weeks. Vitamin D dosage was 500 IU/kg, while SIM dosage was 10 mg/kg. Interleukin-6 (IL-6) concentration and markers of oxidative stress including malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), and reduced glutathione(GSH) concentrations in serum were determined using ELISA kits and spectrophotometry methods, respectively. Results: Treatment with vitamin D or SIM could significantly reduce IL-6 and MDA and increases SOD, GPx activities, and GSH levels. Oxidative stress can result not only from increased ROS production but also from dysfunctional antioxidant defenses. Conclusion: From the experimental results, it was observed that SIM and vitamin D could attenuate oxidative stress and inflammation markers associated with obesity.

Highlights

Obesity is a serious problem posing a considerable threat to human health (Ni et al, 2020)
At the end of the first week, the animals were randomly divided into four groups: (1) control group (Control n=10, fed with standard diet), (2) high-fat diet (HFD) group ( HFD n=10 was fed with HFD), (3) vitamin D group, (4) SIM group (SIM n=10 fed with HFD and treated with SIM 10 mg/kg/day)
A significant rise was detected in vitamin D and SIM groups versus the HFD group; an increase was detected in the SIM group compared to the HFD group and (Fig.2)

Summary

Introduction

Obesity is a serious problem posing a considerable threat to human health (Ni et al, 2020). Obesity is characterized by excessive fat deposition into adipose tissue and non-adipose tissues (Kelishadi et al, 2017). Another characteristic feature coupled with obesity is the alteration of the redox state accompanied by metabolic risk factors (Warolin et al, 2014). Obesity and its related metabolic disorders are strongly linked to oxidative stress. This oxidative status is closely associated with proinflammatory cytokine secretion, which can initiate oxidative stress in a continuous circle (Biswas, 2016). Systemic oxidative stress and inflammation are critical factors in the pathogenesis of obesity-related diseases (Crujeiras et al, 2013)

Objectives

Methods

Results