Abstract
BackgroundObesity is associated with numerous metabolic and inflammatory disorders. The current study was aimed to evaluate the effects of vitamin D administration on the markers of oxidative stress and inflammation in the cardiac tissue of high-fat diet induced obese rats.MethodsIn the beginning of the study, 40 male Wistar rats were divided into two groups: normal diet (ND) and high fat diet (HFD) for 16 weeks; then each group subdivided into two groups including: ND, ND + vitamin D, HFD and HFD + vitamin D. Vitamin D supplementation was done for 5 weeks at 500 IU/kg dosage. Tumor necrosis factor (TNF)-α concentration and markers of oxidative stress including glutathione peroxidase (GPx), superoxide dismutase (SOD), malondialdehyde (MDA) and catalase (CAT) concentrations in the cardiac tissue and serum concentrations of lipids in rats were determined using ELISA kits and spectrophotometry methods respectively.ResultsAccording to our results, GPx activity in ND and ND + vitamin D group was significantly higher compared with HFD group. Similarly, SOD activity was also significantly increased in ND + vitamin D group compared with ND and HFD groups. Moreover, vitamin D administration, significantly reduced catalase activity in ND + vitamin D and HFD + vitamin D groups (P < 0.05). TNF-α concentration in heart tissue in ND + vitamin D group significantly reduced compared with ND group. Cardiac tissue MDA concentration in baseline or after vitamin D administration did not changed significantly.ConclusionVitamin D improved cardiac oxidative stress and inflammatory markers in HFD induced obese rats. Further studies in human models are needed to further confirm the use of this nutrient in daily clinical practice.
Highlights
Obesity is associated with numerous metabolic and inflammatory disorders
glutathione peroxidase (GPx) activity in normal diet (ND) and ND + vitamin D group was significantly higher compared with high fat diet (HFD) group
superoxide dismutase (SOD) activity significantly increased in ND + vitamin D group compared with ND and HFD groups
Summary
The current study was aimed to evaluate the effects of vitamin D administration on the markers of oxidative stress and inflammation in the cardiac tissue of high-fat diet induced obese rats. The prevalence of obesity is increasing alarmingly worldwide and is a major health problem as a main leading cause of morbidity and mortality [1]. High fat diet, is a potent inducer of oxidative stress by altering oxygen metabolism. The fatty deposits as a consequence of high fat diet are vulnerable to suffering oxygen reactions; if the production of these ROS exceeds antioxidant system capacity of the cells, the lipid peroxidation occurs and the lipid peroxidation directly contributes in developing atherosclerosis [8]. It will be worth to investigate that which kind of dietary interventions will possibly reduce the markers of oxidative damage and inflammation in patients with cardiac events
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