EFFECTS OF VITAMIN D ADMINISTRATION IN THE MOUSE MODEL OF POLYCYSTIC OVARIAN SYNDROME

Abstract

To investigate effects of vitamin .D(Vit.D) administration in patients with polycystic ovary syndrome(PCOS) Experimental animal study. Forty female pre-pubertal mice were randomly divided into 4 groups: the control, PCOS, PCOS+low dose Vit.D and PCOS+high dose Vit.D groups(N=10 per group). The PCOS mouse model was developed by 6mg/kg/day dehydroepiandrosterone(DHEA) administration with subcutaneously injections and high fat diet feeding. After 30 days, Vit.D was administrated by intraperitoneal injection in the following 40 days, 130ng/100g/week 1,25(OH)2D3 in low dose Vit.D group, and 1300ng/100g/week 1,25(OH)2D3 in high dose Vit.D group. Controls were injected with vehicle alone and fed with normal diet. At the end of the 70 days, blood samples were collected and the ovarian and liver tissues were taken. All the mice in PCOS+high dose Vit.D group died in two weeks after Vit.D administration. In the other three group, the weight of the PCOS mice was significantly higher than the weight of the controls before Vit.D administration(31.10±2.52, 31.76±2.54 VS 28.82±1.83g, PCOS group, PCOS+low dose Vit.D group VS control group, P=0.022). However, at the end of the study, the weight of the mice in PCOS group was significantly higher than those in control group and PCOS+low dose Vit.D group (41.41±3.90 VS 35.50±2.50, 34.55±2.31 g, P=0.000). The serum 25(OH)D concentration was significantly higher in PCOS+low dose Vit.D group than in control group and PCOS group (99.29±18.31 VS 19.55±4.10,18.04±6.51 ng/ml,P=0.000). The testosterone levels in PCOS group were significantly higher than those of control group and PCOS+low dose Vit.D group (1.27±0.27 VS 0.95±0.15 ,0.90±0.17 ng/ml, P=0.001). Furthermore, total cholesterol levels in control group were lower than in PCOS and PCOS+low dose Vit.D group (3.08±0.44 VS 4.55±0.47, 4.42±0.45mmol/L, P=0.011). Moreover, the ratio of liver weight to body weight was significantly different among the three groups (0.045±0.0046 VS 0.036±0.0043 VS 0.041±0.0031, control group VS PCOS group VS PCOS+low dose Vit.D group, P=0.000). Our results indicate that low dose Vit.D has positive effects on the hormonal changes and obesity observed in PCOS, maybe through liver metabolism regulation, and high dose Vit.D administration may be harmful.