Effects of UGT1A9-1818T ＞ C single nucleotide polymorphism on sedation with propofol

Abstract

Objective To investigate the effects of UGT1 A9-1818T ＞ C single nucleotide polymorphism on sedation with propofol.Methods 150 ASA Ⅰ or Ⅱ female patients,aged 20-50 years,scheduled for elective excision of benign breast lumps under general anesthesia,were enrolled in this study.The polymorphic sites of the UGT1A9-1818T ＞ C allele were analyzed by using polymerase chain reaction and gene sequencing technology.The patients were assigned to one of 3 groups according to their genotypes:wild homozygote group,mutation heterozygote group and mutation homozygote group.Anesthesia was induced by propofol target controlled infusion (TCI) with serum concentration of 3 mg/L,until the patients lost consciousness,intravenous remifentanil and cis-atracurium.The LMA and mechanical ventilation were recorded.Anesthesia was maintained by propofol TCI (3 mg/L),remifentanil continuous infusion,intermittent injection of cis-atracurium.The time,effect-site concentration and bispectral index (BIS) values were recorded when the OAA/S of patients was 4 after stopping the infusion of propofol,and the time and effect-site concentration were recorded when BIS value was 80.Sedation effects of propofol among all groups were compared by using ANOVA.Results There was large individual differences in the propofol sedative effects,but no significant difference was found among the three groups.Conclusion UGT1 A9 -1818T ＞ C SNP site is not the genetic factor that contributing to the individual variation in propofol sedation effects. Key words: Propofol; Uridine diphosphate glucuronosyl tansferase; Single nucleotide polymorphism; Target controlled infusion