Effects of UDP glucuronosyltransferase 1A9 I399 C ＞ T single nucleotide polymorphism on postoperative analgesia with propofol in patients undergoing breast surgery

Abstract

Objective To investigate the effects of UDP glucuronosyhransferase （UGT） 1A9 I399 C 〉 T single nucleotide polymorphism on postoperative sedation with propofol in patients undergoing breast surgery. Methods One hundred and fifty-two ASA I or Ⅱ female patients, aged 20-50 yr, weighing 50-70 kg, scheduled for elective benign breast tumor excision under general anesthesia, were enrolled in this study. The polymorphic sites of the UGT1A9 I399 C 〉 T allele were analyzed by polymerase chain reactionestriction fragment length polymorphism. The patients were assigned to one of 3 groups according to their genotypes： wild homozygote （C/C） group, mutation heterozygote （C/T） group and mutation homozygote （T/T） group. During induction and maintenance of anesthesia, propofol was given by target-controlled infusion with the plasma concentration （Cp） of 3 tLg/ml. Blood samples were taken at 60 min after target-controlled infusion of propofol was started for determination of the Cp of propofol using high-performance liquid chromatography. The time when OAAS was 4 after stopping the infusion of propofol was recorded and the BIS value and effect-site concentration of propofol were also recorded at this time. The time when BIS value was 80 was recorded and the effect-site concentration of propofol was also recorded at this time. Results Genotyping analysis revealed that genotype distribution of UGT1 A9 1399 C 〉 T polymorphism wasC/C 24 cases, C/T 96 cases and T/T 32 cases. The T allele frequency was 53 %. The C allele frequency was 47.4%. There was no significant difference in the Cp of propofol, time when OAAS was 4, BIS value and effectsite concentration of propofol when OAAS was d, time when BIS value was 80 and effect-site concentration of propotbl when BIS value was 80 among the three groups （ P 〉 0.05）. Conclusion UGT1 A9 I399C 〉 T single nucleotide polymorphism is not the＇ genetic factor contributing to the individual variation in the patient s response to postoperative analgesia with propofol in patients undergoing breast surgery. Key words: Propofol ; Conscious sedation ; Glucosyltransferases ; Polymorphism, single nucleotide