Effects of U75302, blocker of leukotriene B4 receptor 1, on cellular immunity and inflammation in septic mice

Abstract

Objective To observe the influence of U75302,blocker of leukotriene B4receptor 1,on immune function in septic mice,so as to explore the implication of blocking leukotriene B4 receptor 1 for treatment of sepsis. Methods Experimental sepsis model was induced by cecal ligation and puncture(CLP).A total of 18male C57BL/6mice were randomly divided into sham group(n=6),CLP group(n=6)and CLP+ U75302intraperitoneal injection group(n=6).The peripheral blood cytokine tumor necrosis factor-α(TNF-α)and interleukin-10(IL-10)levels,peritoneal lavage fluid Gr-1+cell count and thymus T lymphocytes apoptosis were detected in mice of three groups 24hafter surgery.Results Compared with the CLP group,CLP + U75302intraperitoneal injection group had significantly reduced blood TNF-αlevel(by 43%,P 0.05),significantly increased IL-10level(by 88%,P0.05),signficantly decreased Gr-1+cell counts in peritoneal lavage fluid(P0.05),and significantly decreased apoptosis of thymus T lymphocytes(P0.01).Conclusion Blocking leukotriene B4receptor 1with U75302may decrease peripheral TNF-αlevel,increase IL-10level,and improve cellular immunity,which may be involved in inflammation of sepsis.