The protective effect of leukotriene B4 receptor 1 antagonist on sepsis mice

Abstract

Objective To explore the effect of leukotriene B4 receptor 1 (BLT1) antagonist (U-75302) on sepsis mice.Methods Experimental sepsis model was induced by cecal ligation and puncture (CLP).Eighteen male C57BL/6 mice were randomly divided into Sham group (n=6),CLP group (n=6) and CLP+U-75302 intraperitoneally group (n=6).Mice blood,peritoneal lavage fluid,liver and lung were harvested 24 h after operation to determinate blood and peritoneal cavity bacterial burden,liver and lung pathological detection respectively.Twenty four male C57BL/6 mice were randomly divided into sham group (n=8),CLP group (n=8) and CLP+U-75302 intraperitoneally group (n=8).Mice were observed for 7 d survival rate.Results CLP-operated C57BL/6 mice treated with U-75302 intraperitoneally showed lower bacterial burden compared with CLP group (P＜0.01),bacterial burden in peripheral blood and peritoneal lavage fluid reduced by 74％ and 68％ respectively.The injury score of liver and lung were reduced by 62％ and 47％ respectively compared with CLP group(P＜0.01).The 7 d survival rate increased by 16％ compared with CLP group (P＜0.05).Conclusions BLT1 antagonist (U-75302) could protect mice from sepsis,but the mechanism remains to be investigated. Key words: Leukotriene B4; Leukotriene B4 receptor 1; Sepsis; U-75302