Effects of two disinfection/sterilization methods for dentin specimens on dentin permeability.

Abstract

To investigate the effects of two disinfection/sterilization methods on the permeability of dentin specimens. Forty intact human third molars were freshly extracted and cut, close to the pulp chamber, into dentin disks with a 500-μm thickness. The disks were randomized (n = 20 each) into a 70% ethanol group (acid-etched dentin disks soaked in 70% ethanol for 15min) and a steam autoclaving group (acid-etched dentin disks autoclaved for 25min). The permeability (Lp) of each dentin disk was measured before and after either treatment using a hydraulic device, and intra- and inter-group differences in values before and after treatment were analyzed using t tests. Field emission scanning electron microscopy (FE-SEM) micrographs of the dentin surface were acquired and examined. FE-SEM samples were prepared using the critical point drying (CPD) method. Immersion in 70% ethanol increased the Lp values of dentin specimens by 17%, which was not statistically significant. Steam autoclaving significantly reduced dentin permeability by 66% because the dentin collagen mesh became compact and collapsed, as detected by FE-SEM. The disinfection of acid-etched dentin disks using 70% ethanol for 15min does not significantly affect dentin permeability, whereas sterilization of acid-etched dentin disks via autoclaving significantly reduces dentin permeability. Considering the influences of dentin permeability by disinfection/sterilization methods, the disinfection of the acid-etched dentin disks using 70% ethanol for 15min could be used for the study related to dentin permeability, while the sterilization of autoclaving could not.