Abstract
The effects of CGA-64250, a triazole inhibitor of fungal growth, on the lipid composition of Taphrina deformans (D1) were examined using Chromatographic and, in some cases, mass spectrometric techniques. Beginning 8–10 h after inoculation, the total lipid content of cells treated with 0.073 μg/ml averaged 30% more than controls. This was accounted for in part by sterols, which comprised over 2.3 times more of the total lipid from treated cells than from controls. Brassicasterol (ergosta-5,22-dienol) was the principal sterol of T. deformons, at 82% of the total sterol fraction. Ergosterol (ergosta-5,7,22-trienol) was not detected. Methyl sterols such as lanosterol and 24-methylene-dihydrolanosterol represented about 70% of the total in CGA-64250-treated cells, suggesting that this triazole inhibitor blocks the C-14 demethylation of lanosterol. Other responses that appear to be common in fungi treated with C-14 demethylation inhibitors were also observed in CGA-64250-treated T. deformans cells. This included an increased free fatty acid content by a factor of 6.8, and higher degree of lipid unsaturation, as indicated by C 18:1 C 18:2 +C 18:3 ratios of 0.2–0.4 compared to 2.1–2.7 for non-treated cells.
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