Effects of transdiaphragmatic heart massage on the expressions of Fas/Fas-L of cardiac muscle during resuscitation for cardiac arrest in rabbits

Abstract

Objective To compare two modalities of cardiopulmonary resuscitation (CPR) for cardiac arrest with regard to the expressions of Fas/Fas-L and apoptosis of cardiomyocytes. Method A total of rabbits were made into models of cardiac arrest (CA) by asphyxia during laparotomy and then divided equally into two groups as per different modalities of resuscitation employed, transdiaphragmatic heart massage (group A) and conventional heart massage (group B). These rabbits were sacrificed and cardiac tissues were taken out for determination of Fas/Fas-L expressions and apoptosis cardiac muscle cells 6 hours after successful resuscitation. The expressions of Fas/Fas-L were assayed with, and apoptotic cells were counted with. Results The survival rate within 6 hours af-ter successful resuscitation was higher in group A than that in group B (75% vs. 25%, P ＜ 0.05). The expres-sions of Fas/Fas-L [Fas: (27.3 ±4.7) vs. (55.1±6.7);Fasl: (32.7±5.4) vs. (58.9±7.2)] and rate of apoptotic cells [(12.7±3.4)% vs. (22.5±5.2)%] were lower in group A than those in group B(P＜0.05). Conclusions The transdiaphragmatic heart massage for resuscitation of cardiac arrest is more effective than con-ventional one that it is proved by higher survival rate, and lower rates of Fas/Fas-L expressions and apoptotic cells. Key words: Diaphragmatkmiscle; Cardiopulmoriaiyresuscitation; Apoptosis; Ischemical Reperfusion Injury