Abstract
Mitochondria that will esterify inorganic phosphate with high efficiency can be prepared from rat spleen. When mitochondria are prepared from spleens of x-irradiated rats (4 hours after 1000 r), no effect on oxidative phosphorylation is apparent. Under experimental conditions that are unfavorable for efficient phosphorylation, such as suboptimal concentration of cytochrome c, the effect of radiation is manifested by further impairment. The data indicate, however, that the impairment is the result of a change in the composition of the mitochondrial preparation, subsequent to a shift in the distribution of cell populations in the irradiated spleen. Consequently, the mitochondrial preparations from tissues of irradiated rats contain larger amounts of phosphatases, the effect of which will be to catalyze the hydrolysis of organic phosphates, notably ADP and ATP, unless their concentrations are kept low by maintenance of efficient phosphor-ylation and trapping. The activity of the phosphatases is not completely suppressed by the presence of fluoride. (auth)
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