Features of the Distribution of Mast Cell Populations in Lungs and Spleen During Simulated Hypoxic Hypoxia

Abstract

The aim of research was to study the distribution of mast cell populations in the lungs and spleen depending on their expression of proteases in normal conditions and different periods of simulated hypoxic hypoxia.Material and methods. Hypoxic hypoxia was modeled on 87 outbred male rats using 200 liter chambers. The animals were under experimental conditions for four months. Rats were removed from the experiment after 30, 60, 90 and 120 days. After the animals were removed from the experiment, the lungs and the spleen were removed and fixed in 10% neutral buffer solution of Labiko formalin. Lung and spleen preparations were stained with hematoxylin and eosin and according to Van Gieson. For immunohistochemical studies, a panel of monoclonal antibodies was used: Anti-Mast Cell Tryptase antibody, Anti-Mast Cell Chymase antibody.Results. When modeling hypoxia in the structures of the lungs at the terms of 30 and 60 days, the content of predominantly tryptase-positive cells increased, and by the 90th and 120th days, the content of chymase-positive cells increased. By the end of the experimental exposure, the number of tryptase-positive cells increased by 3 times, and chymase-positive – by 7,7 times compared with the control. As the duration of the experiment increased, the presence of tryptase-positive cells in the spleen increased by 3,5 times, chymase-positive cells – by 7 times in the structures of the red and white pulp.Conclusion. During the formation of chronic hypoxic hypoxia in the body of laboratory animals, there is a redistribution of mast cells expressing tryptase and chymase. The most significant increase in the number of chymase-positive mast cells was noted both in the lungs and in the spleen.