Effects of Three Animal Oils on Acute Gastric Mucosal Injury in Rats

Abstract

Objective: To study the effects of three kinds of animal fats on gastric mucosa damage induced by ethanol in rats. Methods: Low (500 mg/kg) and high doses (850 mg/kg) of deer oil, butter and lard were given to rats by gavage for 30 days. Except for the normal group, rats in the other groups were given 1.0 mL of absolute ethanol per rat. One hour later, blood was taken from anesthesia, and gastric tissue was taken. Morphological observation, histopathological examination and the determination of acute injury indexes were performed on the gastric mucosa of rats. Detect the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in serum of rats in each group. The ELISA method was used to determine the serum levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and RT-PCR was used to detect erythropoietin (EPO) and erythropoietin receptor (EPOR) mRNA expression level. Results: Compared with the model group, the weight of rats in each administration group did not change significantly in each period. The stomach weight/body mass of the rats in the deer oil pretreatment group was extremely significantly reduced(P butter>lard. Deer oil has a significant protective effect on acute gastric mucosal injury in rats. The protective effect of butter is weak, while lard has no obvious protective effect.